适体
抗生素
DNA
纳米技术
电化学
材料科学
化学
分子生物学
生物
遗传学
电极
物理化学
作者
Jingyi Cai,Jun Ma,Yuan Dang,Shuangli Chen,Jieli Tang,Sha Yu,Yuanzhen Zhou
摘要
The antibiotics residue pollution in food and environmental matrix has posed a serious threat to global public health. It is an urgent demand in developing highly sensitive methods for the detection of low-abundance antibiotics in real samples. Herein, we have established a magnetic DNA walker-engineered electrochemical aptamer sensor by taking advantages of the distinct recognition capability of aptamer and the excellent signal amplification performance of magnetic DNA walker for the accurate detection of kanamycin (Kana). The aptamer can specifically recognize Kana, while the magnetic DNA walker not only can amplify the target signal through the cascade displacement reaction, but also can effectively reduce the background interference via the magnetic separation, thus ensuring the sensitivity and accuracy of Kana detection in complex samples. The proposed electrochemical sensing method possesses a good linear relationship in the range of 10 fM -1 μM, and the limit of detection is as low as 0.02 fM. Moreover, the excellent selectivity allows this sensor has the ability to specifically identify the Kana from other antibiotics and even in the milk.
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