Recent advances on paper-based microfluidic devices for bioanalysis

Paper-based microfluidic devices have emerged as powerful platforms for bioanalysis offering advantages over other substrate materials including simpler fabrication protocols, compatibility with multi-purpose detectors, and global affordability. This review presents and discusses important information related to manufacturing methods, integrative sensing materials, the clinical relevance of biomarkers and bioanalysis. Examples of applications involving invasive and non-invasive samples, such as human serum, plasma, saliva, sweat, and urine are highlighted. Also, this review covers examples of (bio)sensing applications including the detection of viruses, microorganisms, neurodegenerative, cancer, diabetes, and allergic diseases. Lastly, the review summarizes recent examples of paper-based wearable devices and hybrid sensors dedicated to clinical applications and challenging points that can be carefully observed for the next generation of paper-based microfluidic sensors.