High-performance liquid chromatography using diode array detector and fluorescence detector for hydrogen peroxide analysis in processed fishery foods

Hydrogen peroxide (H2O2) is a food additive for bleaching and sterilization, owing to its strong oxidizing effect. The current study aimed to develop analytical methods to detect trace amounts of residual H2O2 in diverse foods using high-performance liquid chromatography (HPLC) equipped with diode array detector (DAD) or fluorescence detector (FLD). The vanadium(V)-peroxo complex, derived from the reaction of H2O2 with ammonium metavanadate, was used for the detection of H2O2 with DAD. H2O2 was indirectly analyzed using FLD via the detection of 7-hydroxycoumarin, derived by Fenton reaction, followed by verification using liquid chromatography with tandem mass spectrometry analysis. Both the detection methods showed good linearity with R2 > 0.997. Limit of detection and limit of quantification were 0.30 and 0.91 mg/L (8.82 and 26.76 μM) with HPLC–DAD and 0.001 and 0.003 mg/L (0.03 and 0.09 μM) with HPLC–FLD, respectively. Applicability of both the methods was successively tested through sample analysis.