HROB Induces Lung Adenocarcinoma Progression via ZC3HC1‐CCNB1 Axis Regulation and Cell Cycle Dysregulation

ABSTRACT Lung adenocarcinoma (LUAD), a leading cause of cancer‐related mortality, remains a significant global health challenge due to limited understanding of its molecular mechanisms. HROB, a recently identified gene, has been implicated in cell cycle regulation, but its role in lung cancer progression is poorly understood. In this study, we demonstrate that HROB suppresses LUAD progression by interacting with ZC3HC1 and reducing its phosphorylation at Ser354. This de‐phosphorylation event facilitates K27‐linked ubiquitination of CCNB1, promoting its proteasomal degradation and impairing the G2‐to‐M phase transition. Consequently, HROB suppresses cell proliferation and tumor growth. Our findings reveal a novel HROB–ZC3HC1–CCNB1 regulatory axis, providing mechanistic insights into LUAD progression. These results highlight HROB as a potential therapeutic target, offering new avenues for clinical intervention in lung cancer treatment.