诱导多能干细胞
生物
细胞分化
转录因子
神经干细胞
神经科学
神经元
定向微分
祖细胞
细胞
细胞生物学
胚胎干细胞
干细胞
遗传学
基因
作者
Cheng Wang,Jonas Cerneckis,Yanhong Shi
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 141-155
标识
DOI:10.1007/978-1-0716-3810-1_12
摘要
Human-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited accessibility of primary human neurons as well as species-specific divergence between human and rodent brain tissues, hiPSC-derived neurons have become a popular tool for studying neuronal biology in a dish. Here, we provide methods for transcription factor-driven directed differentiation of neurons from hiPSCs via a neural progenitor cell (NPC) intermediate. Doxycycline-inducible expression of neuron fate-determining transcription factors neurogenin 2 (NGN2) and achaete-scute homolog 1 (ASCL1) enables rapid and controllable differentiation of human neurons for disease modeling applications. The provided method is also designed to improve the reproducibility of human neuron differentiation by reducing the batch-to-batch variation of NPC differentiation and lentiviral transduction.
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