The aim of this thesis was to collect and analyze data on aquatic plants within the Herbarium Croaticum collection, the largest and oldest herbarium collection in Croatia. In total 951 herbarium sheets representing 72 species were renovated, digitized, geo-referenced and made publically available in two databases: Flora Croatica Database and Virtual herbarium Herbarium Croaticum. For each species the distribution map was made. Aquatic plants within collection have been collected during 165 years, and avarage age of herbarium sheets is 76 years. Most of the specimens were collected in Croatia, and smaller part in neighboring and geographically close states. The importance of collection is expressed through 11 species, which only evidence of existing in Croatia is this herbarium. Collection of aquatic plants in Herbarium Croaticum is important source of data considering their historical and recent distribution, which are the basis for estimation of distribution changes, threath evaluation and protection planning.