毛细管电泳
协议(科学)
聚合酶链反应
鉴定(生物学)
生物技术
转基因生物
食品科学
计算生物学
医学
生物
遗传学
分子生物学
基因
病理
植物
替代医学
作者
Aaron Burrell,Chester L. Foy,Malcolm Burns
出处
期刊:Biotechnology Research International
[Hindawi Limited]
日期:2011-03-06
卷期号:2011: 1-8
被引量:18
摘要
Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients.
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