甘草苷元
莲藕
大豆黄酮
染料木素
异甘草素
异黄酮
异黄酮素
脱水酶
生物合成
生物化学
泛菌
葛根
化学
生物
酶
类黄酮
基因
中医药
替代医学
16S核糖体RNA
抗氧化剂
突变体
病理
内分泌学
医学
作者
Mie Shimamura,Tomoyoshi Akashi,Nozomu Sakurai,Hideyuki Suzuki,Kazuki Saito,Daisuke Shibata,Shin Ichi Ayabe,Takeshi Aoki
出处
期刊:Plant and Cell Physiology
[Oxford University Press]
日期:2007-09-28
卷期号:48 (11): 1652-1657
被引量:47
摘要
Hairy root cultures of a model legume, Lotus japonicus, were established to characterize two heterologous cDNAs encoding enzymes involved in isoflavone biosynthesis, i.e. licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, showing that HID is a critical determinant of isoflavone productivity. Production of coumestrol in all the genotypes and isoliquiritigenin/liquiritigenin in IFS + HID-overexpressing lines was also noted. These results provide insight into the regulatory mechanism that controls isoflavonoid biosynthesis.
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