微量注射
爪蟾
信使核糖核酸
细胞生物学
核糖核酸
化学
胚胎
分子生物学
生物
基因
生物化学
作者
Hazel Sive,Robert M. Grainger,Richard M. Harland
出处
期刊：CSH Protocols
[Cold Spring Harbor Laboratory Press]
日期：2010-12-01
卷期号：2010 (12): pdb.prot5538-pdb.prot5538
被引量：6
摘要
Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) is a powerful technique for studying development, including protein expression during development. This protocol describes a method for the preparation of secreted (35)S-labeled proteins following mRNA microinjection into Xenopus oocytes.
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