子宫腺肌病
医学
寄生菌
神经生长因子
子宫内膜异位症
免疫组织化学
神经纤维
泌尿科
内科学
内分泌学
解剖
受体
作者
Arisa Takeuchi,Kaori Koga,Masaki Miyashita,Tomoko Makabe,Fusako Sue,Miyuki Harada,Tetsuya Hirata,Yasushi Hirota,Tomoyuki Fujii,Yutaka Osuga
标识
DOI:10.1016/j.ejogrb.2016.10.053
摘要
Objectives To evaluate the in vivo effect of dienogest on proliferation, apoptosis, aromatase expression, vascular density, nerve growth factor (NGF) expression and nerve fiber density in human adenomyosis tissue. Study design Twelve women who underwent hysterectomy for adenomyosis were enrolled. Six patients received dienogest treatment prior to hysterectomy (dienogest group), and age-matched six patients who had not received any hormonal treatment for ≥3 months before surgery (control group). Cell proliferation, vascular and nerve fiber density in adenomyosis tissue were evaluated by staining for Ki67, von Willebrand factor and PGP9.5, respectively. Apoptosis was detected using the TUNEL assay. The expression aromatase and NGF were evaluated by staining for corresponding antibodies. Results The proportion of Ki67 positive epithelial cells was significantly lower in samples from dienogest-treated patients in comparison with controls (p < 0.05). The density of blood vessels in adenomyosis was marginally lower in the dienogest group in comparison with controls but statistical significance was not reached (p = 0.07). The intensity of NGF expression and the density of nerve fibers were significantly lower in the dienogest group compared with controls (p < 0.05 for both). Conclusion This study demonstrates that adenomyosis, taken from patients treated with dienogest, shows remarkable histological features, such as reductions in proliferation, NGF expression and nerve fiber density. These findings indicate the impact of dienogest on local histological events, and explains its therapeutic effect on adenomyosis.
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