Screening and identification of myxobacterias with antifungal activity.

[Objective] The paper was to screen myxobacteria with antifungal activity,and identify its species.[Method] 237 myxobacteria were isolated and purified from 200 copies of soil samples collected from 23 provinces in China.The strains were fermented in four different media,and the fermentation products were extracted by methanol to obtain metabolites.With Candida albicans as the model,the secondary metabolites were carried out high-throughput screening.[Result] A strain of myxobacteria numbered ZJ2 was obtained after secondary screening,its secondary metabolites had activities against C.albicans.16S rDNA cloning and sequencing results showed that ZJ2 was Myxococcus fulvus.[Conclusion] The secondary metabolites of ZJ2 strain had bioactive substances with significant inhibition effect against the growth of C.albicans,which had potential pharmaceutical research and development value.