骨形态发生蛋白6
骨形态发生蛋白15
卵母细胞
内科学
内分泌学
男科
卵泡发生
卵泡期
生物
免疫印迹
窦卵泡
骨形态发生蛋白7
骨形态发生蛋白
细胞生物学
胚胎
医学
胚胎发生
遗传学
基因
作者
Yu Liu,Mei Chen,Xiaopeng Zhao,Xiaohan Ren,Shumin Shao,M. Zha,Ling Zhang
标识
DOI:10.1080/14647273.2019.1660003
摘要
Bone morphogenetic protein 6 (BMP6) is a regulatory peptide secreted by oocytes and granulosa cells that locally regulates folliculogenesis and follicular development. To determine BMP6 location, we studied BMP6 expression in human follicles using immunohistochemistry, real-time polymerase chain reaction (RT-PCR) and western blot analysis. RT-PCR was performed on 354 individual cumulus cell (CC) masses from 48 women to investigate the relationship between BMP6 mRNA expression in CCs and oocyte developmental potential. Results showed that BMP6 protein was mainly located in oocytes from preantral follicles and in granulosa cells from antral follicles. BMP6 mRNA expression was much higher in oocytes than in CCs and mural granulosa cells (mGCs) from preovulatory follicles (p < 0.01), and BMP6 protein level was higher in CCs than in mGCs (p < 0.05). BMP6 mRNA expression was higher in CCs from immature oocytes than in those from mature oocytes (p < 0.05). However, BMP6 mRNA expression in CCs was not associated with oocyte fertilization, embryo morphological grading, or implantation. In conclusion, BMP6 was mainly expressed in oocytes at all human follicular developmental stages and BMP6 mRNA expression in CCs may be negatively correlated with oocyte maturation. BMP6 expression could therefore be used as a biomarker of oocyte maturation.
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