福克斯O1
氧化应激
成骨细胞
化学
骨质疏松症
去卵巢大鼠
西妥因1
内分泌学
超氧化物歧化酶
内科学
白藜芦醇
活性氧
细胞凋亡
骨吸收
下调和上调
细胞生物学
生物
医学
蛋白激酶B
生物化学
体外
雌激素
基因
作者
Yixuan Jiang,Wenqiong Luo,Bin Wang,Xinyu Wang,Ping Gong,Xiong Yu
出处
期刊:Life Sciences
[Elsevier]
日期:2020-04-01
卷期号:246: 117422-117422
被引量:68
标识
DOI:10.1016/j.lfs.2020.117422
摘要
This study aimed to investigate the effects of resveratrol (3, 4', 5-trihydroxystilbene, RES) on osteoporosis and the role of SIRT1/FoxO1 pathway in the process.In vivo, mice were divided into 3 groups, Sham, ovariectomized (OVX) and OVX-RES group. Micro-CT, histology and histomorphometry were conducted to detect details of bone mass and microstructure. The expression of osteoblast markers was tested by Real-time qPCR and serum markers which reflected bone formation and resorption were analyzed by enzyme-linked immunosorbent assay (ELISA). Besides, we assayed sirtuin 1 (SIRT1) expression and the concentration of serum superoxide dismutase (SOD). In vitro, osteoblasts were seperated into 3 groups: control, H2O2 (hydrogen peroxide, H2O2) and H2O2-RES group. Cell proliferation, differentiation and apoptosis were detected. In addition, we tested intracellular reactive oxygen species (ROS) formation and SOD activity detection of osteoblasts. The SIRT1, acetylated FoxO1 (Ac-FoxO1) and nuclear FoxO1 (Nu-FoxO1) expression were detected by western blot.Results revealed that RES could ameliorate bone loss and promote osteogenesis by reinforcing resistance of oxidative stress in OVX mice. RES enhanced proliferation, differentiation and suppressed apoptosis of H2O2-treated osteoblasts. In this process, SIRT1 was upregulated and the level of Nu-FoxO1, which had high transcriptional activity to regulate redox balance, significantly increased.Oxidative stress plays a crucial role in osteoporosis. RES can reinforce resistance to oxidative damage and hence promote osteogenesis via the activation of SIRT1/FoxO1 signaling pathway, which provides a new idea for the prevention and treatment of osteoporosis.
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