Hepatitis B core-specific memory B cell responses associate with clinical parameters in patients with chronic HBV

HBcAg 乙型肝炎表面抗原 埃利斯波特 免疫学 记忆B细胞 抗体 乙型肝炎 B细胞 医学 病毒学 乙型肝炎病毒 慢性肝炎 T细胞 免疫系统 病毒
作者
Thomas Vanwolleghem,Zwier M.A. Groothuismink,Kim Kreefft,Magdeleine Hung,Nikolai Novikov,André Boonstra
出处
期刊:Journal of Hepatology [Elsevier]
卷期号:73 (1): 52-61 被引量:32
标识
DOI:10.1016/j.jhep.2020.01.024
摘要

Little is known about the frequency, phenotype and function of HBV-specific B cells during chronic infection. Here we study HBcAg and HBsAg-specific B cells in different clinical phases of a chronic HBV infection.We included 118 treatment naïve and 34 nucleos(t)ide analogue-treated patients with chronic HBV and 23 healthy HBsAg-vaccinated controls. Global and HBV-specific B lymphocytes were examined by FACS using fluorescently labeled HBsAg and HBcAg as baits. Functional HBV-specific B cell responses were quantified in B cell ELISPOT assays. Anti-HBs and anti-HBc antibodies were measured in serum and in ELISPOT supernatant by ELISA.Higher HBcAg-directed B cell responses were found in HBV clinical phases with elevated vs. low serum alanine aminotransferase (ALT) levels, irrespective of the HBeAg-status. In contrast, HBsAg-directed responses were lower and did not significantly fluctuate. In individual patients a mean 17.8-fold more circulating B cells target HBcAg than HBsAg baits. These HBcAg-specific B cells present a classical memory B cell profile and have slightly higher CD69 expression levels compared to global memory B cells. Viral suppression and ALT normalization upon treatment led to a numeric and functional reduction of HBcAg-specific B cell responses, accompanied by progressive decreases in serum anti-HBc antibodies.HBcAg-specific memory B cells present a classical memory B cell phenotype, vary in number and function throughout HBV's natural history and are significantly reduced during antiviral treatment.In recent years, studies examining the role of B cells during chronic hepatitis B virus infection have regained interest. We show that circulating B cells more often target the hepatitis B core antigen than the hepatitis surface antigen. Moreover, these hepatitis B core-specific B cells associate with the natural history of chronic HBV, and their responses decline during effective antiviral treatment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
btcat完成签到,获得积分10
5秒前
丹青完成签到 ,获得积分10
8秒前
pphss完成签到,获得积分10
11秒前
Jack80发布了新的文献求助200
12秒前
zgtmark完成签到,获得积分10
13秒前
温暖完成签到 ,获得积分10
22秒前
碧蓝雁风完成签到 ,获得积分10
24秒前
Kidmuse完成签到,获得积分10
27秒前
困困困完成签到 ,获得积分10
30秒前
ylkk完成签到 ,获得积分10
41秒前
nuliguan完成签到 ,获得积分10
41秒前
43秒前
晚街听风完成签到 ,获得积分10
58秒前
franca2005完成签到 ,获得积分10
59秒前
kxdxng完成签到,获得积分10
1分钟前
婳祎完成签到 ,获得积分10
1分钟前
喵了个咪完成签到 ,获得积分10
1分钟前
烽烽烽发布了新的文献求助30
1分钟前
skippy完成签到 ,获得积分10
1分钟前
yy完成签到 ,获得积分10
1分钟前
t铁核桃1985完成签到 ,获得积分10
1分钟前
dhdhg完成签到 ,获得积分10
1分钟前
wguanmc完成签到,获得积分10
1分钟前
苏子轩完成签到 ,获得积分10
1分钟前
最美夕阳红完成签到,获得积分10
1分钟前
幽默的谷梦完成签到,获得积分10
1分钟前
如意竺完成签到,获得积分10
1分钟前
想飞的熊完成签到 ,获得积分10
1分钟前
属实有点拉胯完成签到 ,获得积分10
1分钟前
别具一格完成签到 ,获得积分10
1分钟前
simpleblue完成签到 ,获得积分10
2分钟前
wx完成签到 ,获得积分10
2分钟前
论文侠完成签到 ,获得积分10
2分钟前
楚襄谷完成签到 ,获得积分10
2分钟前
Hubery完成签到 ,获得积分10
2分钟前
jbear完成签到 ,获得积分10
2分钟前
Rosaline完成签到 ,获得积分10
2分钟前
tmobiusx完成签到,获得积分10
2分钟前
shame完成签到 ,获得积分10
2分钟前
小刘恨香菜完成签到 ,获得积分10
2分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052652
求助须知:如何正确求助?哪些是违规求助? 2709874
关于积分的说明 7418267
捐赠科研通 2354453
什么是DOI,文献DOI怎么找? 1246090
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921