Nasopharyngeal carcinoma (nasopharyngeal carcinoma) is one of the most common tumor diseases at present. Due to its special clinicopathological characteristics and strong sensitivity to radiotherapy, it is easy to relapse and even cause distant metastasis after treatment. At present, the screening and diagnosis of nasopharyngeal carcinoma mainly relies on image detection. This detection method shows low sensitivity in the screening and clinical staging of nasopharyngeal carcinoma, so the effect is not ideal. The purpose of this paper is to study the significance of epstein-barr virus DNA concentration detection in the screening of nasopharyngeal carcinoma and clinical staging diagnosis, so as to provide theoretical support for the diagnosis of nasopharyngeal carcinoma. This article first to EB virus, and discusses the specific concept of nasopharyngeal carcinoma (NPC), then simply discusses the EB virus and development of the relationship between nasopharyngeal carcinoma, and the studies of the relationship between the simple illustration, finally analyzed with related experiments EB virus DNA testing in the value of screening and clinical staging diagnosis of nasopharyngeal carcinoma (NPC). The experimental results showed that the basic age composition of the three groups of people in the study was relatively consistent. The average age was 49.6 years old, and the majority of them were males, accounting for 85.4% of the total number of patients. The positive values of vca-iga antibody and epstein-barr virus DNA in different groups were the highest in the nasopharyngeal carcinoma group, with concentrations of 91.6% and 52.1%, respectively. Compared with the traditional detection methods, the detection of EB virus DNA concentration has been significantly improved in the diagnosis speed and effect of nasopharyngeal carcinoma, with the diagnosis speed increased by about 17% and the diagnosis efficiency increased by about 30%.