原生质体
转化(遗传学)
生物
基因组
基因
计算生物学
遗传学
作者
B. Gillian Turgeon,Bradford Condon,Jinyuan Liu,Ning Zhang
标识
DOI:10.1007/978-1-60761-611-5_1
摘要
The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.
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