Novel iterative genome mining and engineering of a bifunctional KvVDH enable selective production of furan carboxylic acids from high-concentration 5-hydroxymethylfurfural

In this study, an optimal mutant M3 (A50S/Y370W) with synergistic enhanced thermostability and activity was obtained, and a biocatalytic process was developed to efficiently and selectively oxidize 90–240 mM HMF into multiple furan carboxylic acids.