适体
化学
检出限
费斯特共振能量转移
荧光
石墨烯
胶体金
注意事项
量子点
生物传感器
检测点注意事项
纳米技术
色谱法
纳米颗粒
生物物理学
生物化学
分子生物学
材料科学
物理
量子力学
生物
医学
护理部
免疫学
作者
Xuan Chen,Xianglian Liu,Chuanyu Zhang,Hao Meng,Biwu Liu,Xueyong Wei
出处
期刊:Talanta
[Elsevier]
日期:2022-11-01
卷期号:249: 123661-123661
被引量:9
标识
DOI:10.1016/j.talanta.2022.123661
摘要
In this paper, we report on a novel fluorescent aptasensor based on aptamers modified by both nitrogen-doped graphene quantum dots (N-GQDs) and gold nanoparticles (AuNPs) for the detection of C-reactive protein (CRP). FRET effect is utilized in our aptasensor by the change of aptamers conformation when binding with the target. An obvious fluorescence quench of the N-GQDs can be observed when CRP appears in the assay due to electron transfer between the donor and accepter. A detection limit of 0.2 ng/mL can be achieved by our sensor in PBS buffer which is much lower than the physiological CRP level in human serum. Also, CRP levels in different patients' serum are tested with our assay. Since our aptasensor is rapid (detection time less than 40 min), one-step and very simple to operate, we believe it has great potential to apply for point-of-care testing (POCT).
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