BuyangHuanwu Decoction (BYHWD) inhibits LPS-induced inflammation in BV2 microglial cells by NF-κB/NLRP3 pathway

Abstract Background: Neuroinflammation has been implicated in the pathogenesis of various neurological and cerebrovascular diseases. Recently, microglia has became a promising target to treat neuroinflammation-related diseases. BuyangHuanwu Decoction (BYHWD), a traditional Chinese medicine recipe, is a representative prescription for the treatment of neuroinflammation, showed great therapeutic potential in neuroinflammation-related cerebrovascular disorders including cerebral ischemia. However, it’s possible mechanism of action remains unclear.Methods: In the present research, we studied the therapeutic effects of BYHWD on LPS-induced inflammatory response in BV2 microglia cells. The inflammatory cytokines include interleukin (IL-6), tumor necrosis factor (TNF-α) and nitric oxide (NO) were detected by commercial kit. The inflammatory-related protein was measured by western blot and immunofluorescence.Results: Our findings showed that BYHWD could significantly reverse LPS-induced morphological changes in BV2 microglial and inhibit the local synthesis of proinflammatory cytokines and free radical via directly regulating NF-κB activity and inflammasome assembly. Our data showed that BYHWT inhibit LPS-induced TNF-α, IL-6, and NO production, Also can inhibit iNOS protein expression and the activation of NF-κB taccording to inhibit NLRP3 activation.Conclusions: In conclusion, our results demonstrated that BYHWD is a potential candidate for treating neuroinflammation-related diseases. Certainly, more comprehensive researches on the precise mechanism of action of BYHWD in cerebrovascular disease is required in future vivo studies.