生物膜
家蝇
微生物学
生物
抗菌肽
结晶紫
下调和上调
先天免疫系统
免疫系统
细菌
转录组
防御素
基因表达
抗菌剂
基因
生物化学
免疫学
麝香
遗传学
植物
幼虫
作者
Bing Wang,Peng-Wei Wei,Yang Yao,Chao-Rong Song,Xu Wang,Yong-Xin Yang,Yang Long,Suwen Yang,Yong Hu,Zhongchao Gai,Jian-Wei Wu,Hongmei Li
标识
DOI:10.1016/j.ijbiomac.2022.03.204
摘要
Antibiotic-resistant bacteria (including MRSA) in the clinic pose a growing threat to public health, and antimicrobial peptides (AMPs) have great potential as efficient treatment alternatives. Houseflies have evolved over long periods in complex, dirty environments, developing a special immune system to overcome challenges in harmful environments. AMPs are key innate immune molecules. Herein, two differentially expressed AMPs, Phormicins A and B, were identified by screening transcriptomic changes in response to microbial stimulation. Structural mimic assays indicated that these AMPs exhibited functional divergence due to their C-terminal features. Expression analysis showed that they had different expression patterns. Phormicin B had higher constitutive expression than Phormicin A. However, Phormicin B was sharply downregulated, whereas Phormicin A was highly upregulated, after microbial stimulation. The MIC, MBC and time-growth curves showed the antibacterial spectrum of these peptides. Crystal violet staining and SEM showed that Phormicin D inhibited MRSA biofilm formation. TEM suggested that Phormicin D disrupted the MRSA cell membrane. Furthermore, Phormicin D inhibited biofilm formation by downregulating the expression of biofilm-related genes, including altE and embp. Therefore, housefly Phormicins were functionally characterized as having differential expression patterns and antibacterial & antibiofilm activities. This study provides a new potential peptide for clinical MRSA therapy.
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