脱羧
辅因子
酶
黄素组
化学
脂肪酸
小球藻
生物化学
氧化还原酶
光合作用
氧化脱羧
催化作用
藻类
生物
植物
作者
Damien Sorigué,Bertrand Légeret,Stéphan Cuiné,Stéphanie Blangy,Solène Moulin,Emmanuelle Billon,Pierre Richaud,Sabine Brugière,Yohann Couté,Didier Nurizzo,Pavel Müller,Klaus Brettel,David Pignol,Pascal Arnoux,Yonghua Li‐Beisson,Gilles Peltier,Fred Beisson
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2017-09-01
卷期号:357 (6354): 903-907
被引量:325
标识
DOI:10.1126/science.aan6349
摘要
Although many organisms capture or respond to sunlight, few enzymes are known to be driven by light. Among these are DNA photolyases and the photosynthetic reaction centers. Here, we show that the microalga Chlorella variabilis NC64A harbors a photoenzyme that acts in lipid metabolism. This enzyme belongs to an algae-specific clade of the glucose-methanol-choline oxidoreductase family and catalyzes the decarboxylation of free fatty acids to n-alkanes or -alkenes in response to blue light. Crystal structure of the protein reveals a fatty acid-binding site in a hydrophobic tunnel leading to the light-capturing flavin adenine dinucleotide (FAD) cofactor. The decarboxylation is initiated through electron abstraction from the fatty acid by the photoexcited FAD with a quantum yield >80%. This photoenzyme, which we name fatty acid photodecarboxylase, may be useful in light-driven, bio-based production of hydrocarbons.
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