Establishment of Selenium-deficiency Mouse Model and Effects of Selenium-deficiency on Mouse Myocardium

Objective To develop selenium-deficient BALB/c mice by feeding with self-made selenium-deficient diet.Methods A selenium-deficient mouse food was prepared with local yeast.Young BALB/c mice were fed with the selenium-deficent diet for over 4 months.Selenium concentrations in the blood,liver,and myocardial tissue of the mice fed with selenium-deficient diet were examined.The myocardial enzymes in the treated mice were examined.The ultrastructures of the treated mice myocardial tissues were also observed.Results The selenium concentration in the mouse food prepared with local yeast was 0.016 mg/kg and the standard of selenium-deficiency.The selenium levels in the serum,liver,and myocardial tissue of BALB/c mice fed with the selenium-deficient diet for 4 months were 0.241 mg/kg,0.219 mg/kg,and 0.187 mg/kg.Swelling mitochondria and heteromorphic nucleus could be observed in the selenium-deficient cardiomyocytes.The level of myocardial enzymes in the sera of the selenium-deficient mice was increased.Conclusions A selenium-deficient mouse diet was successfully developed with local yeast,Selenium-deficient mouse can be prepared by feeding this diet.Selenium-deficiency may cause injuries in cardiomyocyte.