[Sucrose utilization determinants of transposon Tn2555].

The Sac- -derivatives of the plasmid pBRS5.2 containing the sucrose utilization transposon Tn2555 were obtained by using the insertional mutagenesis. The sac mutations were mapped within the transposons DNA fragment of about 3kb. The existence of two linkage groups of the sac mutations was shown by complementation analysis. Among the gene products of Tn2555 the polypeptides of about 58, 36, 27, 14 and 12 kd were found. The data on relation of the sac genes of Tn2555 and the ones of the known plasmid pUR400 of Salmonella typhimurium are discussed.