Study on Cryoprotective Effect of Fetal Ovaries Tissue by Vitrification

Objective To investigate the effects of cryoprotective on morphology and function of fetal ovaries tissue by vitrification preservation.Methods Tetal ovarie's tissue was preserved in improved EG5.5/30 vitrification solutions.After being thawed fetal ovary tissue were examined histologically,and xenotransplantation were conducted to observe the ratio of recovery of oestrous cycle,the beginning days of recovery of oestrous cycle,follicles development,so as to evaluate the cryopretective effect of vitrification preservation.Results There were not significant differences among the vitrification transplanted group and the two cultured transplanted group in the recovery of oestrous cycle.But the recovery of oestrous cycle in vitrification transplanted group was higher than that in fresh transplanted group.The beginning days of recovery of oestrous cycle in vitrification transplanted group were shorter than those of the second and the third group.It came close to that of freshtransplanted group.in the number of follicles,vitrification transplanted group showed no significant differences with the two cultured transplanted group but a significant difference with the fresh transplanted group.After xenografting for 18 weeks,a amount of antral follicles were found in the vitrification transplanted group,but a few antral follicles were found in the two cultured transplanted group.Conclusion Vitrification can effectively preserve fetal ovaries tissue and doesn't affect the further development potential of follicles.