Scavenging effect of water-soluble chitosan on hydroxyl radical

Objective To detect the hydroxyl radical-scavenging effect of water-soluble chitosan by phenanthroline-Fe(Ⅱ)oxidation assay.Methods In Fenton reaction system,ascorbic acid(VC)was used as positive control,and the hydroxyl radical-scavenging effect of water-soluble chitosan with different concentration was tested.Results(1)It showed that the highest rate of hydroxyl radical-scavenging of water-soluble chitosan could be 93.67%;(2)The pseudo-positive scavenging effect on hydroxyl radical of water-soluble chitosan with the highest value of 9.03% was tested specially in the experiment.The rate of hydroxyl radical-scavenging of the chitosan was still up to 74.98%～84.64% when the rate of pseudo-positive scavenging was calibrated.Conclusion Water-soluble chitosan is an appropriate kind of hydroxyl radical-scavenging agent.