Ammonia volatilization of nitrogen fertilizer was studied by nitrogen fertilizer applied quantity and depth at the national field research station of agro-ecosystem of Chinese academy of sciences in Hailun county,Heilongjiang province,China.In this study,there were eight different nitrogen fertilizer applied quantity and four depths.The flux of ammonia volatilization was measured with closed chamber method,then calculating the ammonia volatilization of nitrogen fertilizer from soil.Results showed that urea application accelerated farmland ammonia volatilization,and increasing with fertilizer application increase.When nitrogen application was ammonia volatilization,ammonia volatilization loss of nitrogen added up to 21.68%.Under the same quantity of nitrogen application conditions,ammonia volatilization loss of nitrogen decreased with depth,and was 2.49% at 9 cm depth.There was parabola relationship between ammonia volatilization loss and nitrogen application.We suggested that fertilizer application depth was below 3 cm for soybean,6 cm for maize base fertilizer application and 3 cm for supplement of fertilizer.