Effects of annealing temperature on microstructure and properties of pure copper wires containing unidirectional fibrous crystals fabricated by unidirectional continuous casting process and heavy deformation below recrystallization temperature were investigated. The copper wire was annealed in a vacuum firing furnace for 1 h at 200, 300, 400, and 500C: respectively, then was cooled down in the furnace. By observing the microstructure and analyzing the mechanical properties and electrical conductivity of the copper wires before and after annealing treatment, it is shown that at the annealing temperature below 300C, the microstructure of the wires still remained fibrous crystals without evident changes, the tensile strength decreased slightly, and the elongation increased a little. After annealing at 400C, the wires fully recrystallized and compound twins could be found. After annealing at 500C, the recrystallized grains coarsened. When the annealing temperature was higher than 400C, the tensile strength decreased apparently, and the elongation increased greatly. When the annealing temperature ranges from 200 to 400C, the electrical conductivity increased by a small percentage (lower than 2%).