OBJECTIVE: To study the changes of mucosa irritation of crude and processed Radix Phytolaccae. METHOD: Gastrointestinal experiments on rats and mice and the rabbits' eyes irritation experiments were applied to investigate the changes of mucosa irritation of crude and processed Radix Phytolaccae. RESULT: The mucosa irritation of processed Radix Phytolaccae decreased obviously compared to crude one. CONCLUSION: After being processed with vinegar, the mucosa irritation on Radix Phytolaccae decreased obviously.