4 strains of microorganisms addicted to paraffin wax named YC-1、YC-2、YC-3 and YC-4 are isolated and cultivated from oilfield wastewater and activated sludge of refinery from Yanchang Oilfield,which under the only carbon source 52# paraffin wax.When culture on shaking table for 12 days,getting an wax removal rate of 11.12%、9.21%、9.29% and 7.84%.In screening experiment of culture time for 4 strains of microorganisms at 35℃、135r·min-1,therate reached the highest value in 10 days.Made chromatographic analysis for degradation products of paraffin wax by YC-1 under shaking bottle cultivatation at 35℃、135 r·min-1 for 10 days,showed that YC-1 can degrade 52# paraffin wax effectively.Yc-1 could reduce the wax、segregation and sticky of the crude oil effectively.