The study research on the isolation conditions of endophytic fungi from tea plant.Results showed that 3% Sodium hypochlorite was the best surface sterilization in dealing with the leaf of tea and 5 min was the best duration time of surface sterilization.The PDA Agar Medium was the best medium.According to the characteristics of describing strains,Guignardia sp.and Pestalotiopsis sp.made up the most part of endophytic fungi in tea plant.