Oxalicacid,malicacid and citric acid contents in C3F leaves of flue-cured tobacco in Yunnan Dali were quantitatively determined with GC to study the regional characteristics of main non-volatile organic acids contents and their influence on smoking quality.The results showed are as follows.(1) The averaged contents of nonvolatile organic acids was 39.499mg/g,and the contents of oxalic acid,malic acid and citric acid were 2.012mg/g,25.175 and 2.313mg/g,respectively.(2) The contents of oxalic acid,malic acid and nonvolatile organic acids in Hongda variety were significantly higher than that in other variety,while the contents of citric acid in Hongda variety was significantly lower than that in other variety.(3) the content of malic acid in Hongda variety from dry land was significantly higher than that from paddy land.(4) The contents of malic acid and nonvolatile organic acids in Hongda variety from high altitude were significantly higher than those from medium and lower altitude.(5) The content of nonvolatile organic acids in Hongda variety from Jinshajiang river basin,Lancangjiang river basin and Binghu river basin were significantly higher than that from Honghe river basin.6) The non-volatile organic acids mainy influenced the smoking quality index of irritancy,physiological strength and smoke consistence.