The effects of genotype and embryo development on embryo rescue for distant hybrids of lilies

Taking distant hybrids of Lilium Asian,L.Longiflorum,L.Oriental and L.Trumpet as study materials,the embryo rescue experiments were conducted in different genotypes,embryo states and embryo ages.The results showed that: the obtaining rates of embryo-sacs and germination rate of embryos in different genotypes were obviously different.The germination rates of mature embryos were up to 85%.The germination rates of mature embryo-sacs were between 40%~60%.And germination rates of young embryo-sacs were low,most of them were below 20%.Among in vitro plantlets,the abnormal plantlets from mature embryos,mature embryo-sacs,and young embryo-sacs were also different.The optimal embryo age for embryo rescue of all genotypes was 35~90 days.Due to different genotypes,the obtaining rates of embryo-sacs or embryos had different changing patterns in different embryo ages.