阿尔戈瑙特
生物
雄蕊
水稻
生育率
不育
生物发生
男性生育能力
小RNA
功能(生物学)
细胞生物学
遗传学
植物
人口
RNA干扰
基因
医学
花粉
核糖核酸
环境卫生
作者
Fuyan Si,H. Luo,Chao Yang,Jie Gong,Bin Yan,Chunyan Liu,Xianwei Song,Xiaofeng Cao
标识
DOI:10.1007/s11427-022-2204-y
摘要
Phased small interfering RNAs (phasiRNAs) are abundantly expressed in anthers and linked to environment-related male fertility in grasses, yet how they function under different environmental conditions remains unclear. Here, we identified a rice (Oryza sativa) low temperature-induced Argonaute (AGO) protein, OsAGO1d, that is responsible for generating phasiRNAs and preserving male fertility at low temperature. Loss of OsAGO1d function causes low-temperature male sterility associated with delayed programmed cell death of tapetal cells during anther development. OsAGO1d binds miR2118 and miR2275 family members and triggers phasiRNA biogenesis; it also binds 21-nt phasiRNAs with a 5' terminal U. In total, phasiRNAs from 972 loci are OsAGO1d-dependent. OsAGO1d protein moves from anther wall cells into meiocytes, where it loads miR2275 to produce 24-nt phasiRNAs. Together, our results show that OsAGO1d acts as a mobile signal to fine-tune phasiRNA production and this function is important for male fertility at low temperature.
科研通智能强力驱动
Strongly Powered by AbleSci AI