生物
茉莉酸
拟南芥
拟南芥
脱落酸
超氧化物歧化酶
灰葡萄孢菌
非生物胁迫
生物逆境
植物
水杨酸
氧化应激
生物化学
基因
突变体
作者
Khawla Missaoui,Mohamed Habib Ghorbel,Olfa Jrad,Khaled Masmoudi,Faïçal Brini
标识
DOI:10.1016/j.pmpp.2023.102096
摘要
Lipid transfer proteins (LTPs) are members of the pathogenesis-related protein family 14 (PR-14), believed to play a role in plant defense responses. This study reports the overexpression of TdLTP2, a family 2 LTP from durum wheat, in Arabidopsis thaliana. Transgenic Arabidopsis plants with increased TdLTP2 expression unveiled greater tolerance to salt, abscisic acid (ABA), and salicylic acid (SA) stresses, as well as higher tolerance to oxidative stress when compared to wild type (Wt) plants. The transgenic plants also exhibited significant increases in the accumulation of antioxidant enzymes, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), along with decreased levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2). RT-qPCR analysis of the antioxidant enzymes confirm the role played by TdLTP2 in regulating oxidative stress under salt (100 mM NaCl) and oxidative (3 mM H2O2) stresses. Moreover, detached leaves from transgenic Arabidopsis lines expressing the TdLTP2 gene displayed enhanced resistance against fungal pathogens such as Aspergillus niger, Fusarium graminarium, Botrytis cinerea, and Alternaria solani. Finally, RT-qPCR results for biotic stress-related genes (PR1, PDF1.2, LOX3, and VSP2) showed differential expression in transgenic TdLTP2 lines when plants were treated with jasmonic acid (JA). Collectively, these findings provide evidence for the involvement of TdLTP2 gene in enhancing tolerance to both abiotic and biotic stresses in crop plants.
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