Hepatocyte HSPA12A inhibits macrophage chemotaxis and activation to attenuate liver ischemia/reperfusion injury via suppressing glycolysis-mediated HMGB1 lactylation and secretion of hepatocytes

Rationale: Liver ischemia-reperfusion (LI/R) injury is characterized by two interconnected phases: local ischemia that causes hepatic cell damage to release damage-associated molecular pattern (DAMPs), and DAMPs that recruit immune cells to elicit inflammatory cascade for further injury of hepatocytes.High-mobility group box 1 (HMGB1) is a representative DAMP.Studies in macrophages demonstrated that HMGB1 is secreted after lactylation during sepsis.However, whether lactylation mediates HMGB1 secretion from hepatocytes after LI/R is known.Heat shock protein A12A (HSPA12A) is an atypical member of HSP70 family.Methods: Gene expression was examined by microarray analysis and immunoblotting.The hepatic injury was analyzed using released ALT and AST activities assays.Hepatic macrophage chemotaxis was evaluated by Transwell chemotaxis assays.Inflammatory mediators were evaluated by immunoblotting.HMGB1 secretion was examined in exosomes or serum.HMGB1 lactylation was determined using immunoprecipitation and immunoblotting.Results: Here, we report that LI/R decreased HSPA12A expression in hepatocytes, while hepatocyte-specific HSPA12A overexpression attenuated LI/R-induced hepatic dysfunction and mortality of mice.We also noticed that hepatocyte HSPA12A overexpression suppressed macrophage chemotaxis to LI/R-exposed livers in vivo and to hypoxia/reoxygenation (H/R)-exposed hepatocytes in vitro.The LI/R-increased serum HMGB1 levels of mice and the H/R-increased HMGB1 lactylation and secretion levels of hepatocytes were also inhibited by hepatocyte HSPA12A overexpression.By contrast, HSPA12A knockout in hepatocytes promoted not only H/R-induced HMGB1 lactylation and secretion of hepatocytes but also the effects of H/R-hepatocytes on macrophage chemotaxis and inflammatory activation, while all these deleterious effects of HSPA12A knockout were reversed following hepatocyte HMGB1 knockdown.Further molecular analyses showed that HSPA12A overexpression reduced glycolysis-generated lactate, thus decreasing HMGB1 lactylation and secretion from hepatocytes, thereby inhibiting not only macrophage chemotaxis but also the subsequent inflammatory cascade, which ultimately protecting against LI/R injury. Ivyspring