生物
核糖核酸
小RNA
计算生物学
RNA编辑
遗传学
非编码RNA
降级(电信)
细胞生物学
基因
电信
计算机科学
作者
Federico Fuchs Wightman,Jerónimo Lukin,Sebastián Giusti,Michael Soutschek,Laureano Bragado,Berta Pozzi,María L Pierelli,Paula Valeria Gonzalez,Juan Pablo Fededa,Gerhard Schratt,Rina Fujiwara,Jeremy E. Wilusz,Damián Refojo,Manuel de la Mata
摘要
Abstract A subset of circular RNAs (circRNAs) and linear RNAs have been proposed to ‘sponge’ or block microRNA activity. Additionally, certain RNAs induce microRNA destruction through the process of Target RNA-Directed MicroRNA Degradation (TDMD), but whether both linear and circular transcripts are equivalent in driving TDMD is unknown. Here, we studied whether circular/linear topology of endogenous and artificial RNA targets affects TDMD. Consistent with previous knowledge that Cdr1as (ciRS-7) circular RNA protects miR-7 from Cyrano-mediated TDMD, we demonstrate that depletion of Cdr1as reduces miR-7 abundance. In contrast, overexpression of an artificial linear version of Cdr1as drives miR-7 degradation. Using plasmids that express a circRNA with minimal co-expressed cognate linear RNA, we show differential effects on TDMD that cannot be attributed to the nucleotide sequence, as the TDMD properties of a sequence often differ when in a circular versus linear form. By analysing RNA sequencing data of a neuron differentiation system, we further detect potential effects of circRNAs on microRNA stability. Our results support the view that RNA circularity influences TDMD, either enhancing or inhibiting it on specific microRNAs.
科研通智能强力驱动
Strongly Powered by AbleSci AI