神经毒性
SH-SY5Y型
转录组
下调和上调
小桶
生物
信号转导
细胞生物学
神经母细胞瘤
神经发生
小RNA
基因表达
生物途径
基因
化学
毒性
生物化学
遗传学
细胞培养
有机化学
作者
Deepa Gandhi,Saravanadevi Sivanesan,Krishnamurthi Kannan
标识
DOI:10.1007/s12011-017-1153-5
摘要
Manganese (Mn) is an essential trace element required for many physiological functions including proper biochemical and cellular functioning of the central nervous system (CNS). However, exposure to excess level of Mn through occupational settings or from environmental sources has been associated with neurotoxicity. The cellular and molecular mechanism of Mn-induced neurotoxicity remains unclear. In the current study, we investigated the effects of 30-day exposure to a sub-lethal concentration of Mn (100 μM) in human neuroblastoma cells (SH-SY5Y) using transcriptomic approach. Microarray analysis revealed differential expression of 1057 transcripts in Mn-exposed SH-SY5Y cells as compared to control cells. Gene functional annotation cluster analysis exhibited that the differentially expressed genes were associated with several biological pathways. Specifically, genes involved in neuronal pathways including neuron differentiation and development, regulation of neurogenesis, synaptic transmission, and neuronal cell death (apoptosis) were found to be significantly altered. KEGG pathway analysis showed upregulation of p53 signaling pathways and neuroactive ligand-receptor interaction pathways, and downregulation of neurotrophin signaling pathway. On the basis of the gene expression profile, possible molecular mechanisms underlying Mn-induced neuronal toxicity were predicted.
科研通智能强力驱动
Strongly Powered by AbleSci AI