化学
哑铃
连接器
DNA
小RNA
DNA连接酶
多重位移放大
检出限
分子生物学
碱基对
聚合酶链反应
纳米技术
生物物理学
计算生物学
生物化学
基因
生物
DNA提取
色谱法
操作系统
材料科学
生理学
计算机科学
作者
Abdu Ahmed Abdullah AL‐maskri,Guangbo Jin,Yang Li,Jadera Talap,Marwan Almoiliqy,Apu Chowdhury,Su Zeng,Ying Zhou,Sheng Cai
出处
期刊:Talanta
[Elsevier]
日期:2022-11-01
卷期号:249: 123618-123618
被引量:1
标识
DOI:10.1016/j.talanta.2022.123618
摘要
Based on self-assembly amplification, we designed a novel microRNA (miRNA)-detection method with high specificity and sensitivity. Two unique DNA probes named Linker A and Linker B were modified with phosphorothioate (PS) at both ends. In the presence of the target miRNA, these two DNA probes were ligated together by T4 DNA ligase enzyme to form a dumbbell-shaped DNA. Then the dumbbell-shaped structure would be extended with Bst 2.0 DNA polymerase enzyme, triggering the strand displacement activity without using additional primers. These results revealed our method's ultralow detection limit (300 fM), excellent selectivity, simple operation, and capability to discriminate single-base mismatches. It is believed that this proposed approach would have great application potential in clinical diagnosis and other involved fields.
科研通智能强力驱动
Strongly Powered by AbleSci AI