Evaluation of Maternal, Embryo, and Placental Effects in CD-1 Mice following Gestational Exposure to Perfluorooctanoic Acid (PFOA) or Hexafluoropropylene Oxide Dimer Acid (HFPO-DA or GenX)

PFOA/GENX Study Results and Discussion PlacentasIn this study placentas from the right uterine horn were taken for histology and the left side was frozen by Fenton lab.Five slides were cut for each cassette (2 sections per slide) and the 1 st and last slides stained with H&E.The intervening 3 slides may be used for special stains or immunohistochemistry. R on necropsy notes for "right" is denoted as "P" on histology slides.So R1=P1, R2-P2, etc.All aspects of the placentas were evaluated including the labyrinth, decidua and chorion.The metrial gland and yolk sac were also evaluated when present.If a placenta from the left horn had a gross lesion it was also collected for histopathological evaluation.Many of the lesions seen in this study were dam effects, affecting a majority or all of the embryos, although number of placentas per group are listed below.Most of the placentas from E11.5 vehicle controls were within normal limits.Two embryos shared a placenta.2 out of 38 were abnormal.The two figures below show the range of normal for the labyrinth from this group of vehicle controls.The focus was on the trilaminar trophoblasts based on the atrophy seen in the treated groups.E11.5 vehicle control 114-07 P2.Normal labyrinth with thick trilaminar trophoblast layer (20X).