Pathogenicity and Virulence of Isaria javanica, Metarhizium anisopliae, and Beauveria bassiana Strains for Control of Drosophila suzukii1 (Matsumura)

Pathogenicity and virulence of three strains of entomopathogenic fungi, Isaria javanica (CHE-CNRCB 307), Metarhizium anisopliae (CHE-CNRCB 224), and Beauveria bassiana (CHE-CNRCB 168), and a check of 1 ml of sterile 0.03% Tween® 80 were evaluated on adult spotted-wing drosophila, Drosophila suzukii (Matsumura). Lethal concentration 50 (LC50) of entomopathogenic fungi was determined at five concentrations (1 × 104, 1 × 105, 1 × 106, 1 × 107, and 1 × 108 conidia per ml-1) and the lethal time (LT50) at a dose of 1 × 108 conidia per ml. The evaluated strains had different amounts of pathogenicity and virulence on adult D. suzukii. The I. javanica (CHE-CNRCB 307) strain had the largest significant differences with LC50 of 1.6 × 104 adult conidia per ml and LT50 of 4,579 days. The M. anisopliae (CHE-CNRCB 224) and B. bassiana (CHE-CNRCB 168) strains were not significantly different compared to each other, with LC50 of 3.1 × 105 and 1.4 × 106 adult conidia per ml and LT50 of 18.16 and 15.3 days, respectively. Results provide information about entomopathogenic fungi and support for using it to control D. suzukii.