化学
色谱法
人绒毛膜促性腺激素
串联质谱法
尿
萃取(化学)
洗脱
定量分析(化学)
促性腺激素
亲和层析
质谱法
生物化学
激素
酶
作者
Lay‐Harn Gam,Sock-Ying Tham,Aishah A. Latiff
标识
DOI:10.1016/s1570-0232(03)00264-2
摘要
A confirmatory and quantitative HPLC-tandem mass spectrometry (MS-MS) method for human chorionic gonadotropin hormone (hCG) at concentrations as low as 5 IU/l following immunoaffinity extraction of the glycoprotein from urine was developed. The extraction method involved retention of urinary hCG in the immunoaffinity column via specific antigen-antibody interaction. A variety of eluents were then used to quantitatively elute hCG from the immunoaffinity column. Qualitative and quantitative analysis of hCG were undertaken using MS-MS by identifying the amino acid sequence of the marker peptide betaT5 obtained from hCG by tryptic digestion and the peak areas of three product ions b(6)(+), b(9)(+) and y(11)(+), respectively.
科研通智能强力驱动
Strongly Powered by AbleSci AI