间充质干细胞
移植
细胞生物学
生物
病理
体内
医学
癌症研究
间质细胞
创伤性脑损伤
外科
遗传学
精神科
作者
Chi-Jen Hung,Chao‐Ling Yao,Fu‐Chou Cheng,Ming-Hsien Wu,Tzu‐Hao Wang,Shiaw-Min Hwang
出处
期刊:Cytotherapy
[Elsevier]
日期:2010-07-01
卷期号:12 (4): 455-465
被引量:32
标识
DOI:10.3109/14653240903555827
摘要
Abstract
Background aims
Human mesenchymal stromal cells (hMSC) play a crucial role in tissue engineering and regenerative medicine, and have important clinical potential for cell therapy. However, many hMSC studies have been restricted by limited cell numbers and difficult detection in vivo. To expand the lifespan, hMSC are usually immortalized by virus-mediated gene transfer. However, these genetically modified cells easily lose critical phenotypes and stable genotypes because of insertional mutagenesis. Methods
We used a non-viral transfection method to establish human telomerase reverse transcriptase-immortalized cord blood hMSC (hTERT-cbMSC). We also established red fluorescent protein (RFP)-expressing hTERT-cbMSC (hTERT/RFP-cbMSC) by the same non-viral transfection method, and these cells were injected into a rat model with traumatic brain injury for in vivo detection analysis. Results
The hTERT-cbMSC could grow more than 200 population doublings with a stable doubling time and maintained differentiation capacities. hTERT/RFP-cbMSC could proliferate efficiently within 2 weeks at the injury location and could be detected easily under a fluorescent microscope. Importantly, both hTERT-cbMSC and hTERT/RFP-cbMSC showed no chromosomal abnormalities by karyotype analysis and no tumor formation in severe combined immunodeficient (SCID) mice by transplantation assay. Conclusions
We have developed immortalized cbMSC with hTERT expression and RFP expression, which will be useful tools for stem cell research and translational study.
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