化学
内吞作用
内化
固体脂质纳米粒
血脑屏障
跨细胞
药物输送到大脑
药物输送
网格蛋白
并行传输
流式细胞术
生物物理学
载脂蛋白E
纳米颗粒
药理学
纳米技术
生物化学
细胞
神经科学
分子生物学
膜
中枢神经系统
生物
医学
疾病
材料科学
有机化学
磁导率
病理
作者
Ana Rute Neves,Joana Fontes Queiroz,Sofia A. Costa Lima
标识
DOI:10.1021/acs.bioconjchem.6b00705
摘要
Several strategies have been implemented to enhance brain drug delivery, and herein solid lipid nanoparticles functionalized with apolipoprotein E were tested in hCMEC/D3 cell monolayers. The mean diameter of 160 nm, negative charge of −12 mV, and their lipophilic characteristics make these nanosystems suitable for brain delivery. Confocal images and flow cytometry data showed a cellular uptake increase of 1.8-fold for SLN-Palmitate-ApoE and 1.9-fold for SLN-DSPE-ApoE when compared with the non-functionalized SLNs. Clathrin-mediated endocytosis was distinguished as the preferential internalization pathway involved in cellular uptake and nanoparticles could cross the blood-brain barrier predominantly by a transcellular pathway. The understanding of the mechanisms involved in the transport of these nanosystems through the blood-brain barrier may potentiate their application on brain drug delivery.
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