ISOLATION AND PURIFICATION OF HIRUDIN FROM HIRUDINARIA MANILLENSIS IN GUANG XI, CHINA

Frontiers on Separation Science and Technology, pp. 523-528 (2004) No AccessISOLATION AND PURIFICATION OF HIRUDIN FROM HIRUDINARIA MANILLENSIS IN GUANG XI, CHINACHUN-GU LIU, ZHANG-FA TONG, YOU-YAN LIU, AI-ZU LEI and YU-CAI HECHUN-GU LIUSchool of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China, ZHANG-FA TONGSchool of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China, YOU-YAN LIUSchool of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China, AI-ZU LEISchool of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China and YU-CAI HESchool of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, Chinahttps://doi.org/10.1142/9789812702623_0101Cited by:0 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail Abstract: Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech, Hirudo medicinalls. Hirudin has been intensively investigated for research and therapeutic purpose. It's one of the most powerful anticoagulants. A specific extraction method from the salivary gland secretions of the alive leechs, Hirudinaria manillensis, had been applied. The purified hirudin can be obtained by precipitation procedures followed by an anion exchange chromatography on DEAE-C52 and a gel filtration chromatography on Sephadex-G25. The hirudin was analyzed by the method of titration with thrombin. The hirudin activity in salivary gland secretions is 5 AT-U/mL. The production recovery rate is 16.2% and ratio activity is 7140 AT-U/mL protein. FiguresReferencesRelatedDetails Frontiers on Separation Science and TechnologyMetrics History PDF download