Glycosidase is one of the member of glycoside hydrolases,its character and function is most fascinating topics.The research includes α-mannosidase,Arab-glucosidase,β-xylosidase,β-D-glucuronidase,β-fructofuranosidase,chitotriosidase,high temperature β-mannosidase and thioglycoligase common glycosidase and its the basic characteristics.Preliminary review of glycosidase mechanism of research methods,modification of glycosidase and biological adaptation of glycosyl compounds the basic strategies and methods.