The effect of ammonia-N on immune activity of Ruditapes philippinarum

By the acute toxic experiment on Ruditapes philippinarum of ammonia-N,the related equation between the concentration of ammonia-N and the mortality of R.philippinarum was y =1.827 1x+0.651 9,R2=0.971 9.According to this equation,it was calculated that the half death in 24 h of R.philippinarum was about 239.88 mg/L,the concentration of ammonia-N which caused 5% death in 24h was 13.49 mg/L.By the experiment on the influence of ammonia-N on the number of haemocyte and the bacteriolytic activity,it was found that the number of haemocytes of R.philippinarum decreased as increasing the concentration of ammonia-N in different times,and their difference was remarkable(FF0.05).As the time increasing,there was the same change trend of the number of haemocyte of R.philippinarum.It reached minimum when reacted for 6h,after which it became increased.The number of haemocyte had no significant difference in the 12h and 24h(FF0.05) reaction times.