小胶质细胞
生物
体内
海马结构
表型
神经科学
神经胶质
肿瘤坏死因子α
细胞生物学
免疫学
中枢神经系统
炎症
基因
遗传学
作者
Annette Masuch,Rianne van der Pijl,Lisa Füner,Yochai Wolf,Bart J. L. Eggen,Erik Boddeke,Knut Biber
出处
期刊:Glia
[Wiley]
日期:2016-05-05
卷期号:64 (8): 1285-1297
被引量:25
摘要
Recent data suggest that ramified microglia fulfil various tasks in the brain. However, to investigate this unique cell type cultured primary microglia are only a poor model. We here describe a method to deplete and repopulate organotypic hippocampal slice cultures (OHSC) with ramified microglia isolated from adult mouse brain creating microglia-replenished OHSC (Mrep-OHSC). Replenished microglia integrate into the tissue and ramify to a degree indistinguishable from their counterparts in the mouse brain. Moreover, wild-type slices replenished with microglia from TNFα-deficient animals provide similar results as OHSC prepared from microglia-specific TNFα-knockout mice (CX3CR1cre/TNFαfl/fl). Furthermore, this study demonstrates that replenished microglia in OHSC maintain original functions and properties acquired in vivo. Microglia from ERCC1Δ/ko mice, a mouse model of accelerated aging, maintain enhanced Mac2 expression and their activated phenotype after replenishment to wild-type OHSC tissue. Thus, the present study demonstrates that Mrep-OHSC are a unique tool to construct chimeric brain slices allowing studying the function of different phenotypes of in vivo like microglia in a tissue culture setting. GLIA 2016 GLIA 2016;64:1285–1297
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