Extraction of puerarin isoflavones by stirring

Ethanol extraction was used to extract isoflavones from Pueraria Lobata Ohwi.The content of isoflavones was detected by UV spectrophotometry using puerarin as standard sample to draw the standard curve.Based on single factor experiments and orthogonal experiments,the effect on the extraction rate was studied during stirring speed,temperature,extraction time and liquid-solid ratio.The optimal operation conditions are as follows:stirring speed 200 r/min,liquid-solid ratio 10:1,time 60 min,temperature 60 ℃,under this condition puerarin isoflavones extraction yield was 7.12%,transfer rate was 94.5%.