Development of enzyme-linked immune-sorbent assay for detection of malachite green and crystal violet residue in fish

An enzyme-linked immune-sorbent assay(ELISA) method was developed to determine the malachite green(MG) and crystal violet residues in fish and the performance of the ELISA method was evaluated.The results showed that the ELISA kit exhibited a good linearity in the range of 0.2 ng/mL to 5.4 ng/mL,and the 50% competitive inhibition rate was 0.50 ng/mL.The low limit of detection for MG in fish muscle was 0.17 ng/g.For the spiked experiment,the average recovery rate was higher than 72.4%,the coefficient of variations(CV) for intra-assay and inter-assay were lower than 12.4% and 12.9%,respectively.The developed method was sensitive,accurate,rapid and suitable for the determination of malachite green,crystal violet,leucomalachite green and leucocrystal violet total residues in fish.