Based on the long-term conservational tillage experiment of sloping land of Heilongjiang Hailun City,the effect of five tillage treatments: no tillage,minimum tillage,cross ridge,ridge tillage(see as conservational tillage in former) and conventional plowing tillage on the activity of catalase,urease and sucrose were studied.Results showed that activity of catalase of no tillage,minimum tillage,cross ridge and ridge tillage was higher 1.70%-34.21% than that of plowing tillage;activity of urease and sucrose of no tillage and minimum tillage was higher 4.81%-67.94% than that of other tillage treatments;activity of urease and sucrose of cross ridge and ridge tillage was higher 8.97%-36.00% than that of plowing tillage;the highest activity of catalase,urease and sucrose was shown in podding period.No tillage and minimum tillage had more ability to fertilize black soil than cross ridge and ridge tillage,which could protect soil and water,thereby,no tillage and minimum tillage were fit for sloping land.